Vegansk 'yoghurt'. Foto: DTU Fødevareinstituttet

Vegan ‘yogurt’ made with lactic acid bacteria from plants

Bacteria and microorganisms Food, fish and agriculture Food technology Food production

Researchers at DTU have identified lactic acid bacteria from Danish plants, which can be used to make a 100% vegan yogurt alternative with just three ingredients.

Good old-fashioned yogurt is made with cow’s milk and a starter culture that consists of different lactic acid bacteria. At the appropriate temperature, the starter culture begins to break down the sugar in the milk, thereby fermenting the milk and turning it into the thickened, soured product that we know as yogurt.

An increasing global demand for plant-based alternatives to traditional milk products has led researchers at the National Food Institute, Technical University of Denmark (DTU), to create a plant-based yogurt alternative with as few ingredients as possible.

There are already commercially available plant-based yogurt alternatives on the market, which are made with existing starter cultures that were developed for use in milk-based products. In order for the bacteria to grow enough to turn the plant-based products sour and give them a nice aroma, producers have to add sugar.

In addition to the added sugar, the producers use other ingredients to give their products the desired consistency and taste, which results in a longer list of ingredients.

Scanning the plant kingdom for lactic acid bacteria

The National Food Institute researchers had a working hypothesis that using bacteria from the plant kingdom would be most efficient when fermenting a vegetable product such as a soy beverage given that these bacteria are optimized to break down sugar in plant material.

Researchers and students searched through fields and forests in large parts of Denmark in order to collect samples of different plant materials. They then examined the samples in the laboratory to see if they contained lactic acid bacteria with the properties that make the bacteria suitable as a starter culture in a plant-based product.

Through the research-based teaching, the researchers at the National Food Institute have managed to isolate bacterial strains that can acidify  a soy beverage and create a texture that is reminiscent of a traditional yogurt.

Using sidestreams from the production of beer

The researchers have managed to incorporate spent grain—a side stream from the production of beer—in the production of the product. This third ingredient make the resulting product more sustainable and increases its fibre content. 

Depending on how much spent grain is used in the production of the ‘yogurt’, it can also give the final product an aroma, which can help conceal any unwanted flavours from the soy.

The project continues 

The work to develop the product has been carried out with a grant from the EU Innovation Fund Climate-KIC and in collaboration with the companies Carlsberg, Naturli 'and Novozymes.

The researchers are continuing the work on optimizing the consistency and taste of the product with the expectation that a commercial partner will be able to bring it to market.

Vegansk 'yoghurt'. Foto: DTU Fødevareinstituttet

The vegan yogurt alternative is made with just three ingredients: Soy beverage, spent grain and a plant-based starter culture.

Answer questions about attitudes towards plant-based food 

Through a short questionnaire, the National Food Institute hopes to gain an insight into Danes' attitudes towards plant-based foods as well as what consumers would expect from a plant-based food in order to want to eat it. It takes about 5 minutes to answer the questionnaire

Read more 

The focus of the Research Group for Microbial Biotechnology and Biorefining at the National Food Institute is effective bioconversion of a wide range of renewable raw materials. Read more about the group’s work on the institute’s website. 

Read more about the National Food Institute’s work to utilize side streams more efficiently in these other news items from the institute: The waste from seafood to become the sustainable foods of the future, Eating grass is not for ruminants only and Students invent eco-friendly vegan cheese.